Intermediate
Part:BBa_J100007
Designed by: Eric Sawyer Group: Campbell M Lab (2011-05-24)
pLac+RBS+LoxP+Stop Sequence+LoxP
This stop sequence blocks transcription and translation by the formation of a stem-loop structure. In the presence of Cre recombinase, the stop sequence is excised from the DNA. A protein coding part placed downstream of this part will be expressed only in the presence of Cre.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 284
[edit]
Categories
Parameters
None |